Medical science advances at an astonishing pace on all fronts. The area of diagnostics is one particular field where great efforts are made to devise tests and procedures that can help doctors to diagnose diseases quicker and more accurately. This is important because successful treatment often depends upon an early diagnosis. ELISA kits, for example, have empowered doctors to efficiently detect a wide range of diseases.
EIA, as this technique is also known, is an extremely popular test. This is because it is so easy to carry out and as an added bonus large numbers of tests can be done at the same time. The purpose of the test is to find out what concentration of antigens or antibodies exists in a specific sample. Antigen and antibody concentrations are very important indicators in the diagnostic process.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
The administration of the test is straight forward and painless. A small blood sample is taken from the patient. This sample is then sent to the laboratory where the technician will add the blood sample to an antigen already present in a petri dish. If the blood sample contains antibodies to the antigen, the two substances will bind together. Enzymes are added and the reaction between the antibodies and the antigen is observed.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Another important variant of EIA is the indirect test. In this case two stages are engineered by covering the antigens in polystyrene plates. This is done to achieve increased sensitivity. Antibodies are introduced in primary and secondary stages. The test can produce multiple results which means that is saves cost and time. In many cases this test, which is more elaborate, eliminate the need for more tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
EIA, as this technique is also known, is an extremely popular test. This is because it is so easy to carry out and as an added bonus large numbers of tests can be done at the same time. The purpose of the test is to find out what concentration of antigens or antibodies exists in a specific sample. Antigen and antibody concentrations are very important indicators in the diagnostic process.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
The administration of the test is straight forward and painless. A small blood sample is taken from the patient. This sample is then sent to the laboratory where the technician will add the blood sample to an antigen already present in a petri dish. If the blood sample contains antibodies to the antigen, the two substances will bind together. Enzymes are added and the reaction between the antibodies and the antigen is observed.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Another important variant of EIA is the indirect test. In this case two stages are engineered by covering the antigens in polystyrene plates. This is done to achieve increased sensitivity. Antibodies are introduced in primary and secondary stages. The test can produce multiple results which means that is saves cost and time. In many cases this test, which is more elaborate, eliminate the need for more tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
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